An improved polyacrylamide gel electrophoretic method for demonstration of oligoclonal bands of immunoglobulin G in cerebrospinal fluid.
نویسندگان
چکیده
A two-step procedure is described in which analytical disc gel electrophoresis in polyacrylamide is used to demonstrate oligoclonal bands of immunoglobulin G in cerebrospinal fluid. The principal advantage of the procedure is that unconcentrated samples of cerebrospinal fluid as small as 200 fiL can be assayed. The results obtained in samples from 100 patients with various neurologic diseases show that the procedure is sensitive and specific for multiple sclerosis. (
منابع مشابه
Two agarose electrophoretic systems for demonstration of oligoclonal bands in cerebrospinal fluid compared.
Demonstration of oligoclonal bands by electrophoresis of cerebrospinal fluid is an important aid in establishing the diagnosis of multiple sclerosis. Electrophoretic systems vary in their effectiveness in doing so. We compared two systems in this respect. For a thin-layer agarose system, sensitivity was less (47%) than for a high-resolution agarose system (87%). Each system had good specificity...
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To the Editor: In .95% of patients suffering from clinically unambiguous multiple sclerosis (MS), oligoclonal IgG can be detected in cerebrospinal fluid (CSF) (1, 2). Isoelectric focusing is the most sensitive method for detecting oligoclonal bands (OCBs) (1 ), whereas agarose gel electrophoresis is reported to detect OCBs in ;80% of cases (2 ). A commercial electrophoresis unit (Beckman Instru...
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ورودعنوان ژورنال:
- American journal of clinical pathology
دوره 79 1 شماره
صفحات -
تاریخ انتشار 1983